This was followed immediately by the collections of Robert Brown and Matthew Flinders of the survey sloop HMS Investigator in the adjacent Port Phillip Bay at Arthurs Seat a few days later on 27 April as Flinders was charting the southern shores between 26 April and 3 May 1802. The ship’s party included Kew gardener Peter Good and the artist Ferdinand Bauer.
The earlier Flinders and Bass circumnavigation of Van Diemen’s Land (Tasmania) in the Norfolk in 1798–99 had proved that a strait, now Bass Strait, existed between the mainland and Van Dieman’s Land.
On 8 December 1802 John Murray, who had succeeded Grant in command of the Lady Nelson, visited Western Port’s Churchill Island, eating some of the vegetables. Wheat, corn, a few potatoes and two onions had survived. The exact site of the garden and blockhouse is not known.  Further seed was sown in 1826 by Captain Wright who established a Western Port settlement in 1826. 
Gardener-botanists would remain an integral part of exploration and survey teams, both on land and sea. In early 1803 a survey team was sent out in HMS Cumberland under Charles Robbins to assist Surveyor-General Charles Grimes in assessing King Island for settlement, also the lower reaches of the Yarra and Maribyrnong Rivers (English presence, it was assumed, would also discourage French territorial ambitions), and the basalt plains to the west of Port Phillip. The team included convict gardener James Fleming who kept a journal recording his observations of the vegetation, but if plants were collected there is now no record of them. At today’s Dight’s Falls near native huts and fish traps they planted seeds, probably an assortment of wheat, corn, radish, cress and mustard.
Fleming returned to England from Port Jackson on HMS Glatton in 1803 as the gardener in charge of ’19 boxes of Plants and Shrubs’ bound for Banks in London, together with a letter of commendation from Rev. Samuel Marsden to Banks, which introduced Fleming as ‘a good Gardener and Botanist’. Fleming delivered the plants to Banks personally, but Banks was sick in bed. Governor King had also asked Fleming to make a list of all the non-indigenous plants of the colony of New South Wales, adding the names of those that might be of future use. Fleming’s list was duly sent by King to Lord Hobart. Ducker notes that ‘this list of agricultural and garden plants is most astonishing in its diversity . . . surprising . . . that such a variety of fruit trees and garden plants were already in this young colony’ Australia was already playing a role in the globalisation of cultivated plants.